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. 2020 Feb 14;9(2):445. doi: 10.3390/cells9020445

Figure 3.

Figure 3

TGFβR2 is a direct target of miR-202-5p. (A) Targets of miR-202-5p were predicted using three miRNA databases. Nine overlapping genes are shown. (B) TGFβR2 is a target of miR-202-5p in different species, rectangles indicated the binding sites of miR-202-5p. (C) Schematic illustrating the design of luciferase reporters with three miR-202-5p binding sites in wildtype TGFβR2 3′UTR (WT) or mutant TGFβR2 3′UTR (mut). (D) TGFβR2 3′UTR or its mutant luciferase reporter vectors were cotransfected with miR-202-5p mimics (or negative control) into HEK293T cells. Cells were lysed 48 h post-transfection and assessed for relative luciferase activities. (E) Western blotting (WB) was used to analyze the expression of TGFβR1 and TGFβR2. (F) TGFβR2 mRNA levels were detected in GCs transfected with miR-202-5p mimics, inhibitors, mimics NC or inhibitor NC. Experiments were performed on a minimum of three occasions. Bars indicate the mean ± SEM of three independent replicates; *p < 0.05, **p < 0.01, N.S.: Not significant.