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. 2020 Feb 14;9(2):445. doi: 10.3390/cells9020445

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MiR-202-5p mediates the SF1 regulation of canonical TGF-β signaling pathway. (A) SF1 regulates TGFβR2 mRNA expression in GCs. Cells were transfected with pcDNA3.1-SF1 or SF1-siRNA. mRNAs were quantified by qPCR. (B) pcDNA3.1-SF1 and miR-202-5p inhibitors were cotransfected into GCs and protein levels were analyzed by WB. (C) SF1-siRNA and miR-202-5p mimics were cotransfected into GCs and protein levels were analyzed by WB. Experiments were repeated three times. Bars indicate the mean ± SEM of three independent replicates; *p < 0.05, **p < 0.01, N.S.: Not significant.