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. 2020 Feb 22;9(2):504. doi: 10.3390/cells9020504

Figure 3.

Figure 3

miRNA transfection and programming efficiency in MSC. (A) Uptake of miRNA was determined using Cy3-labelled miRNA and flow cytometry. (B) Detection of dead cells revealed low cytotoxicity induced by miRNA transfection. (C) Relative expression of cardiac marker genes among all tested cell types, four weeks after transfection and cultivation under different culture conditions. Reprogramming efficiency with cardiac induction medium I, II and myo-miRNAs (miR-1, miR-499, miR-208, miR-133) resulted in an up-regulation of cardiac specific markers in all types of MSC, while most profound up-regulation was found for cardiac induction medium II. Among tested MSC, the strongest increase of cardiac gene expression was observed for adMSC. Note, no beneficial effects on cardiac programming were observed following myo-miRNA transfection. Data are shown as mean ± SEM, obtained from three donors for each MSC type. Statistical analysis was performed using ANOVA test, followed by Bonferroni post-hoc analysis. * p ≤ 0.5, ** p ≤ 0.05, *** p ≤ 0.001.