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. 2020 Feb 18;9(2):456. doi: 10.3390/cells9020456

Figure 2.

Figure 2

Influence of mesedin on the expression of α-adrenergic receptors in murine hepatic stellate cells (M1-4HSCs). (A–D) Immunofluorescence analysis of α1/α-ARs (red) and α-SMA (green) in M1-4HSCs treated with 10 µM mesedin vs untreated controls (ctrl.). The scale bar corresponds to 100 µm, and the cell nuclei were stained with DAPI. (E,F) Quantification of α1-AR+/α-SMA+ and α2-AR/α-SMA+ M1-4HSCs upon incubation with mesedin vs the control (ctrl). (G) Western blot and densitometric analysis of α2-AR and β2-AR in M1-4HSCs with and without mesedin. Student’s t-test, n = 4, p < 0.05 (*), p < 0.01 (**).