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. 2020 Feb 21;9(2):494. doi: 10.3390/cells9020494

Figure 5.

Figure 5

TLRs mediate redox status in mammary gland during S. uberis infection. (A,B) The protein expressions of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), malondialdehyde (MDA), and uncoupling protein 2 (UCP2) were determined by kits in mammary gland of WTB6, WTB10, TLR2−/−, TLR4−/− mice with or without S. uberis. A is for the protein expressions for TLR2−/− group and B is for the TLR4−/− group. Data are presented as the means ± SEM (n = 6). * (p < 0.05) = significantly different between the indicated groups. (C) CellQuest Pro acquisition and analysis software analyzed the levels of reactive oxygen species (ROS) and mitochondrial reactive oxygen species (mROS) in MECs of Control, siTLR2, siTLR4, and siTLR2/4 groups with or without S. uberis. (D) The activity of UCP2 was determined by ELISA in MECs of Control, siTLR2, siTLR4 and siTLR2/4 groups with or without S. uberis. Data were presented as the means ± SEM (n = 3). * (p < 0.05) = significantly different between the indicated groups. All experiments were repeated three times.