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. 2020 Feb 13;21(4):1243. doi: 10.3390/ijms21041243

Figure 5.

Figure 5

SDS-PAGE analysis of the induction of His-pET32a and rMjPellino protein expression and Western blot analysis of purified His-pET32a, rMjPellino, rVP19, rVP24, rVP26, and rVP28 proteins. (A) His-pET32a protein and rMjPellino expression. M, protein molecular standard; blank, total cellular extract from E. coli BL21(DE3) pLysS chemically competent cells before IPTG induction; 1, SDS-PAGE of pET-32a after IPTG induction; 2, SDS-PAGE of rMjPellino after IPTG induction. (B) Western blot analysis of pET-32a (lane 1) and rMjPellino (lane 2). (C) Western blot analysis of pGEX-4T-1, rVP19, rVP24, rVP26, and rVP28 (from left to right).