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. 2020 Feb 14;21(4):1302. doi: 10.3390/ijms21041302

Figure 4.

Figure 4

GEF-H1 has minimal impact on immune defense against S. typhimurium infection. (A) IL-1β released by LPS-primed WT and GEF-H1-deficient BMDMs infected with S. typhimurim at different MOI. (B) Representative western blot analysis of pro-caspase-1 (Casp1 p45), active-caspase-1 subunit p20 (Casp1 p20), and ASC in the supernatants or cell lysates of LPS-primed BMDMs left unstimulated or infected with S. typhimurim at indicated MOI. (C) LDH released by LPS-primed WT and GEF-H1-deficient BMDMs infected with S. typhimurim (MOI = 10) at different time points. (D) Intracellular CFU was measured by a gentamicin protection assay in LPS-primed WT and GEF-H1-deficient BMDMs infected with S. typhimurim at MOI = 10 for 3 h. n = 3 independent experiments per group. Data represent mean ± SD. Statistical analyses were performed using unpaired two-tailed t-test.