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Observation of the black layer in maize kernels at different developmental stages. (A–E) Longitudinal section of maize kernels 10, 20, 30, 40, and 50 days after pollination (DAP). The arrowhead indicates the position of the black layer. Bar = 2 mm; (F-I) Scanning electron microscopy analysis of the black layer of maize kernels 20, 30, 40, and 50 DAP. Regions surrounded by red boxes indicate the structure of the black layer. Bar = 200 μm.
