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mRNA Wnt pathway signaling-related molecules analyzed at 15, 30, 60 and 120 min. CEMP1-p4 induced higher expression of β-catenin. At 60′ glycogen synthase kinase 3B (GSK3B) increased significantly. The transcription factor T-cell transcription factor (Tcf)/lymphoid enhancer binding factor 1 (Lef1) showed its higher expression at 30′ and 120´. Identical triplicates were prepared for each reaction, and the experiment was repeated 3 times independently (n = 3/group).
