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Expression of mineralization-related markers at the mRNA level. CEMP-1-p4 induced higher expression of core-binding factor subunit alpha-1 (RUNX2) and osterix (OSX) on days 3 and 7. Integrin-binding sialoprotein (IBSP) was highly expressed at the middle phase and osteocalcin (OCN) at the late stages of mineralization, when compared to controls. Identical triplicates were prepared for each reaction, and the experiment was repeated 3 times independently (n = 3/group). *p < 0.05.
