Figure 4.

Accumulation of calnexin and calreticulin in soybean root under flooding with silver NPs/nicotinic acid/KNO₃. Two-day-old soybeans were flooded with or without 5 ppm silver NPs/8 µM nicotinic acid/ 0.1 mM KNO₃ for two days. Proteins (10 µg) extracted from roots were applied to gel electrophoresis and immuno-blot was performed with anti-calnexin and anti-calreticulin antibodies. The integrated densities of bands were calculated using Image J software. Data are presented as the mean ± S.D. from three independent biological replicates. Significance was calculated using Students t-test (* p < 0.05, ** p < 0.01).