Figure 1.

Myogenic differentiation of myoblasts derived from fetal bovine tissue. (A) Microscopic images of bovine myoblasts on days 0, 2, and 4 of differentiation. Scale bars = 100 µm. (B) Transcript levels of MYF5, MYOD, MYOG, MYH1, MYH2, MYH3 and MYH4 during proliferation (P) and myogenic differentiation (D0, D2, and D4). The results are represented as the mean ± the SEM from at least three independent experiments. (C) Western blot evaluating the protein levels of MYOD and MHCs in the cultured cells, as described in (B).