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. 2020 Feb 13;21(4):1248. doi: 10.3390/ijms21041248

Figure 2.

Figure 2

NCAPG KD elongated the prometaphase and metaphase. (A) The silencing efficiency of NCAPG siRNAs. We designed three siRNAs that were transfected into myoblasts, and the mRNA level was measured to determine the siRNA efficiency. The results are represented as the mean ± the SEM (three biological replicates), ** p < 0.01. (B) Cell morphology of NCAPG silencing myoblasts. NCAPG was depleted in the proliferating myoblasts. Scale bar = 100 µm. (C) An analysis of chromosome condensation during the prophase in live myoblasts depleted of NCAPG. To visualize chromatin, we stably expressed H2B–EGFP in the myoblasts. Prophase image sequences were extracted from long-term imaging experiments and aligned along a time axis according to nuclear envelope breakdown (NEBD). Imaging was done 48–72 h after siRNA transfection. Bar = 5 µm. (D) The time duration of the prometaphase and metaphase in the myoblasts. The time from NEBD to the onset of the anaphase was measured using at least six replicates, ** p < 0.01.