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. 2020 Feb 13;21(4):1248. doi: 10.3390/ijms21041248

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Myogenic gene expression was upregulated after 1.5 d of differentiation in NCAPG-silenced myoblast. (A) The cell morphology of NCAPG-silenced myoblasts on day 1.5 of differentiation. Scale bar = 100 µm. (B) Transcript levels of muscle-specific genes on day 1.5 of differentiation. The results are presented as the mean ± the SEM from at least three independent experiments: * p < 0.05, ** p < 0.01. (C) Western blot evaluating the protein levels of MYOD, MYOG, and MHCs in myoblasts on day 1.5 of differentiation.