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. 2020 Feb 13;21(4):1259. doi: 10.3390/ijms21041259
AKT v-akt murine thymoma viral oncogene homolog 1
ALP/Alp alkaline phosphatase
ARS Alizarin red staining
BMSCs bone marrow stromal cells
BMD bone mineral density
BMP bone morphogenetic protein
BV/TV bone volume to tissue volume (bone volume fraction)
CCK-8 Cell Counting Kit-8
CDK2 cyclin-dependent kinase 2
CDK12 cyclin-dependent kinase 12
Ctr control
ColIα1 collagen type 1 alpha-1
EDTA ethylene diamine tetraacetic acid
Erk extracellular signal-regulated kinase
FAK focal adhesion kinase
FBS fetal bovine serum
FNDC5/Fndc5 fibronectin type III domain-containing 5
Gapdh/GAPDH glyceraldehyde 3-phosphate dehydrogenase
MAP mitogen-activated protein
MAR mineral apposition rate
microCT micro-computed tomography
NC negative control
OD optical density
pAb Polyclonal antibody
PBS phosphate buffer saline
PFA paraformaldehyde
PI3K phosphatidylinositol-3 kinase
r-irisin recombinant irisin
ROS reactive oxygen species
RPM random position machine
SM simulated microgravity
Si-β-cat β-catenin siRNA
Si-NC siRNA NC
Vec pcDNA3.1 blank vector
Wnt wingless-type MMTV integration site family
α-MEM alpha Modified Eagle’s Medium
β-cat pcDNA3.1-β-catenin plasmid