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. 2020 Feb 13;21(4):1263. doi: 10.3390/ijms21041263

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Investigation of the cytoskeleton. F-actin visualized by means of phalloidin and immunofluorescence (IF) staining for β-actin. F-actin pictures show (A) 3-day 1 g control, (B) 3-day AD, (C) 3-day MCS, (D) 5-day 1 g, (E) 5-day AD, and (F) 5-day MCS. Big arrows in (B) and (E) point to lamellipodia, notched arrows in (B) point to filopodia, and small arrows in (E) point to stress fibers. β-actin pictures show (G) 3-day 1 g control, (H) 3-day AD, (I) 3-day MCS, (J) 5-day 1 g, (K) 5-day AD, and (L) 5-day MCS. Arrows in (H) point to membrane blebbing. Relative mRNA transcription levels of (M) ACTB and (N) TUBB genes in 3-day and 5-day samples were analyzed by qPCR. * p < 0.05.