AD	Adherent cell(s)
BME	β-mercaptoethanol
BSA	Bovine serum albumin
DAPI	4′,6-diamidino-2-phenylindole
ECM	Extracellular matrix
EMT	Epithelial–mesenchymal transition
ERK	Extracellular signal regulated kinase
FA	Focal adhesion
FAK	Focal adhesion kinase
FLK1	Fms-related tyrosine kinase 1
FLT1	Fetal liver kinase 1
FBS	Fetal bovine albumin
HE	Hematoxylin–eosin
HRP	Horseradish peroxidase
IF	Immunofluorescence
MAPK	Mitogen-activated protein kinase
MCS	Multicellular spheroid(s)
MEK1	Mitogen-activated protein kinase kinase 1
mTOR	Mammalian target of rapamycin
mTORC2	mTOR complex 2
µg	Microgravity
NGAL (LCN2)	Neutrophil gelatinase-associated lipocalin (lipocalin 2)
NTC	No-template control
1 g	Normal gravity
PAM	PI3K/AKT/mTOR
PBS	Phosphate-buffered saline
PBST	Phosphate-buffered saline with Tween
PFA	Paraformaldehyde
PI3K	Phosphoinositide 3-kinase
PKB	Protein kinase B
qPCR	Quantitative real-time polymerase chain reaction
RAF	Rapidly accelerated fibrosarcoma
RICTOR	Rapamycin-insensitive companion of mTOR
RIPA	Radioimmunoprecipitation assay
RPM	Random positioning machine
rpm	Rounds per minute
RWV	Rotating wall vessel
SR	Sirius red
SRC (Src)	Steroid receptor coactivator (proto-oncogene, non-receptor tyrosine kinase)
S-µg	Simulated microgravity
TBST	Tris-buffered saline with Tween
VEGF	Vascular endothelial growth factor
VEGFR	Vascular endothelial growth factor receptor
WHO	World Health Organization
TRIFMA	Time-resolved immunofluorometric assay
2D	Two-dimensional
3D	Three-dimensional