Table 1.
List of primer sequences used in quantitative PCR. All sequences are listed in the 5′–3′ direction.
| Factor | Primer Name | Sequence 5′–3′ |
|---|---|---|
| 18S-rRNA | 18s-F | GGAGCCTGCGGCTTAATTT |
| 18s-R | CAACTAAGAACGGCCATGCA | |
| Actin-beta (ACTB) | ACTB-F | TGCCGACAGGATGCAGAAG |
| ACTB-R | GCCGATCCACACGGAGTACT | |
| RAC-alpha Serine/threonine-protein kinase (AKT1) | Akt1-F | CTTCTATGGCGCTGAGATTGTG |
| Akt1-R | CAGCATGAGGTTCTCCAGCT | |
| Collagen 1 alpha 1 (COL1A1) | COL1A1-F | ACGAAGACATCCCACCAATCAC |
| COL1A1-R | CGTTGTCGCAGACGCAGAT | |
| Collagen 4 alpha 5 (COL4A5) | COL4A5-F | GGTACCTGTAACTACTATGCCAACTCCTA |
| COL4A5-R | CGGCTAATTCGTGTCCTCAAG | |
| E-cadherin (CDH1) | CDH1-F | GCTGGACCGAGAGAGTTTCC |
| CDH1-R | CAGCTGTTGCTGTTGTGCTT | |
| Extracellular signal-regulated kinase 1 (ERK1) | ERK1-F | ACCTGCGACCTTAAGATTTGTGA |
| ERK1-R | AGCCACATACTCCGTCAGGAA | |
| Extracellular signal-regulated kinase 2 (ERK2) | ERK2-F | TTCCAACCTGCTGCTCAACA |
| ERK2-R | TCTGTCAGGAACCCTGTGTGAT | |
| Focal adhesion kinase 1 (Protein-tyrosin kinase 2) (FAK1 (PTK2)) | FAK1-F | TGTGGGTAAACCAGATCCTGC |
| FAK1-R | CTGAAGCTTGACACCCTCGT | |
| Fibronectin (FN1) | FN1-F | TGAGGAGCATGGTTTTAGGAGAA |
| FN1-R | TCCTCATTTACATTCGGCGTATAC | |
| Laminin alpha 3 (LAMA3) | LAMA3-F | AAAGCAAGAAGTCAGTCCAGC |
| LAMA3-R | TCCCATGAAGACCATCTCGG | |
| Laminin β2 (LAMB2) | LAMB2-F | TGCTCATGGTCAATGCTAATCTG |
| LAMB2-R | TCTATCAATCCTCTTCCTTGGACAA | |
| Mitogen-activated protein kinase kinase 1 (MAP2K1) | MAP2K1-F | CGTTACCCGGGTCCAAAATG |
| MAP2K1-R | TCCAAGTTGGTCTCCGCA | |
| Mechanistic target of rapamycin kinase (MTOR) | MTOR-F | ATCTTGGCCATAGCTAGCCTC |
| MTOR-R | ACAACTGGGTCATTGGAGGG | |
| Neutrophil gelatinase-associated lipocalin (NGAL, LCN2) | LCN2-F | AGGGAGTACTTCAAGATCACCCTCTA |
| LCN2-R | AGAGATTTGGAGAAGCGGATGA | |
| Raf-1 proto-oncogene, serine/threonine kinase (RAF1) | RAF1-F | GGGAGCTTGGAAGACGATCAG |
| RAF1-R | ACACGGATAGTGTTGCTTGTC | |
| Rapamycin-insensitive companion of MTOR (RICTOR) | RICTOR-F | GGAAGCCTGTTGATGGTGAT |
| RICTOR-R | GGCAGCCTGTTTTATGGTGT | |
| Steroid receptor coactivator-1 (SRC1) | SRC1-F | CCACCTTTGTGGCCCTCTAT |
| SRC1-R | CCTCTGTGTTGTTGACAATCTGG | |
| Talin-1 (TLN1) | TLN1-F | GATGGCTATTACTCAGTACAGACAACTGA |
| TLN1-R | CATAGTAGACTCCTCATCTCCTTCCA | |
| Tubulin-beta (TUBB) | TUBB-F | CTGGACCGCATCTCTGTGTACTAC |
| TUBB-R | GACCTGAGCGAACAGAGTCCAT | |
| Vascular endothelial growth factor A (VEGFA) | VEGFA-F | GCGCTGATAGACATCCATGAAC |
| VEGFA-R | CTACCTCCACCATGCCAAGTG | |
| Vascular endothelial growth factor receptor 1 (FLT1) | FLT1-F | CCCTCGCCGGAAGTTGTAT |
| FLT1-R | GATAATTAACGAGTAGCCACGAGTCAA | |
| Vascular endothelial growth factor receptor 2 (FLK1) | FLK1-F | TCTTCTGGCTACTTCTTGTCATCATC |
| FLK1-R | GATGGACAAGTAGCCTGTCTTCAGT | |
| Vinculin (VCL) | VCL-F | GTCTCGGCTGCTCGTATCTT |
| VCL-R | GTCCACCAGCCCTGTCATTT |