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. 2020 Feb 14;21(4):1304. doi: 10.3390/ijms21041304

Figure 4.

Figure 4

Genotyping of Pinb2 genes using the new PCR method. (a) Diagram describing the workflow of set of PCR primers to genotype Pinb2-7A, Pinb2-7B and Pinb2-7D loci. (b) Primer pair U non-specifically amplified Pinb2 genes encoded by Pinb2-7A, Pinb2-7B and Pinb2-7D loci from several Triticeae species. Sizes of DNA markers used are labeled. Specific PCR products are pointed out by black arrowheads. (c) PCR primer pair B1 specifically detected the Pinb2-7B1-v3 allele in bread and durum wheats (T. aestivum and T. turgidum spp. durum). (d) Nested PCR primer sets B2/B3 specifically detected the Pinb2-7B1-v2 allele in bread wheat (T. aestivum) Chinese Spring. (e) The dCAPS primers-derived PCR amplification and subsequent BstX I digestion distinguished the Pinb2-7B1-v2-1 allele (107 bp) from the other Pinb2-7B1-v2 alleles (135 bp). (f) PCR primer pair D specifically amplified the Pinb2-7D1 gene in bread wheat.