Figure 4.

Iron blocks BMDCs maturation and generates CD11c⁺CD11b⁺F4/80⁺MHCII^low cells. Representative density plots and histograms of BMDCs cultured in iron-overload condition for seven days or for 24 h (A,B). BMDCs cultured in iron-overload mimicking condition for seven days or for 24 h were exposed to LPS for 24 h (C,D). Cells cultured in iron-overload mimicking condition for seven days were treated with vehicle or LPS and analyzed by FACS 24 h later (E–H). Histograms represent MHCII (E), CD80 (F), and CD86 (G) CD11c⁺CD11b⁺F4/80⁺MHCII^low cells surface expression. Bar plot showing the (Mean fluorescence index) MFI of MHCII, CD80, and CD86 (H).