Figure 5.

UV light-enhanced alkaline phosphatase (ALP) activity, an early- to middle-stage marker of osteoblasts, in the OVX and sham groups. (A) Colorimetrically quantified ALP activity standardized per cell at day 7 is shown. (B) ALP activity measured as the ALP-positive (red) area at day 7. Representative images of the stained culture are presented on the top. The ALP-positive area measured from the images is presented at the bottom histogram in square centimeters. Each value represents the mean ± standard deviation of triplicate experiments (n = 3). * p < 0.05, significant difference between untreated and UV light-treated surfaces.