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qRT-PCR validation of the main HT responsive miRNAs identified by small RNA sequencing. (a) Fold-change of miRNAs at the four developmental stages under HT stress based on small RNA sequencing data. (b) The relative expression of the same miRNA members under HT stress detected by qRT-PCR and represented as means of three replicates (n = 3) ± standard error. Significant differences indicated with letters above each bar (p ≤ 0.05).
