Figure 9.

SAA-induced increase in kidney tissue 3-Cl-Tyr levels in young and old cohorts of Apo E-/- mice. Mice were allocated into 4 treatment arms and administered with vehicle (control), LPS, SAA alone, or SAA in combination with HDL. Mice were monitored for 4 weeks (young cohort) or 18 weeks (old cohort) after commencement of treatment, then sacrificed and kidneys were harvested and processed, and the levels of 3-Cl-Tyr were determined by liquid chromatography coupled with mass spectrometry, as described in methods section. Data represent mean ± SD with mice numbers were control (n = 5), LPS (n = 5), SAA (n = 5), and HDL/SAA (n = 5). Different to the respective controls, * p < 0.05, ** p < 0.01; different to the respective SAA and HDL/SAA group, # p < 0.05, ## p < 0.01.