Figure 7.

Effect of SPL-kd on various signaling molecules and transcription factors. Confluent control (ctrl) or SPL-kd HCMEC/D3 cells were rendered serum-free for 4 h prior to stimulation with either vehicle (−), 1 nM TNFα (A) or 50 nM TPA (B) in serum-free DMEM for 10 min. Protein extracts were separated by SDS-PAGE and subjected to Western blot analysis using antibodies against phospho-p38-MAPK, total p38-MAPK, phospho-AMPKα, total AMPKα, phospho-c-Jun, total c-Jun, JunB, phospho-protein kinase C (PKC) substrates, phospho-MARCKS, and α-tubulin. The data show representative blots, out of 3–4 independent experiments, performed in triplicates.