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. 2020 Feb 15;21(4):1317. doi: 10.3390/ijms21041317

Figure 4.

Figure 4

Phosphatidylserine decarboxylase activity is the limiting step in DXR efficiency. (A) The phospholipid composition of mitochondrial membranes was determined on other cells, MRC5, by high-performance thin-layer chromatography and compared to that of HeLa. The quantification of PC, PS, CL, and PE was performed by densitometry and expressed as a percentage of total phospholipids (*, p < 0.05). (B) PSD activity was measured on mitochondrial fractions of MRC5 and compared to those of HeLa cells. The data are expressed in PE-NBD/PS-NBD ratio per minute, as mean values ± SEM of N ≥ 3 experiments (**, p < 0.01). (C) The PS/PE ratio was determined in MRC5 cells treated or untreated with 5 μM DXR. (D) The effect of DXR on the entire mitochondrial membrane composition was assayed. DXR treatment was unable to induce a mitochondrial membrane remodeling within MCR5 cells. (E) The mitochondrial respiration was measured in MRC5 cells treated for 6 h with 5 μM DXR, and endogenous rates of respiration were recorded and compared to the control condition. Data are expressed as mean values of oxygen consumption rates (OCR) ± SEM, N = 5 experiments.