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. 2020 Feb 18;21(4):1364. doi: 10.3390/ijms21041364

Figure 1.

Figure 1

Effects of the exposure to hypoxia on the expression of HIF-1α in SH-SY5Y and IMR-32 NB cells. After incubation times, cells were harvested and used for the analysis of HIF-1α mRNA levels by Real-Time PCR (A); the nuclear content of HIF-1α protein in cells incubated for 4 h in hypoxic condition was assessed by Western blotting (B) and subsequent densitometric analysis (C). Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 significant differences in comparison to controls.