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. 2020 Feb 15;21(4):1309. doi: 10.3390/ijms21041309

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of the ectopically expressed Mdm RING domains on p53 activation. (A) U2OS cells were transfected with FLAG-Mdm2, FLAG-MdmX, FLAG-Mdm2 RING, and FLAG-MdmX RING. The total cell lysate was immunoblotted for the protein levels of full-length Mdm2 and MdmX. (B) U2OS cells were transfected with FLAG-Mdm2, FLAG-MdmX, FLAG-Mdm2 RING, and YFP-MdmX RING. The total cell lysate was immunoblotted for the protein levels of p53, phosphorylated p53 at Ser15, p21, and Bax. (C) U2OS cells were transfected with FLAG-Mdm2, FLAG-MdmX, FLAG-Mdm2 RING, and FLAG-MdmX RING. Relative mRNA expression levels of p21 (CDKN1A) and Bax (BAX) were analyzed. Results are expressed as Mean ± SEM of three biological replicates. Expression levels of p21 and Bax in the vector control are taken as 1. The results were analyzed using statistical one-way ANOVA test. * p ≤ 0.05.