Figure 1.

Performance of polysomal profiling in SW55Ti centrifugation tubes. Identical cell lysates from HEK-293 cells (a) and yeast strain W303 ceg1^ts cultivated either at 24 °C (b) or 37 °C (c) were loaded on 10%–50% sucrose gradients prepared either in SW55Ti tubes (left panels) or SW41Ti tubes (right panels). RNA was isolated from equal fractions of the respective profiles and loaded on agarose gel (lower panels). Individual fraction numbers are indicated next to each electrophoretogram. Note in upper polysome profiles the obtained HEK-293 curves are shifted up by 200 mV to prevent overlapping with the yeast derived data curves. Arrows indicate the position of specific peaks within the profiles: i.e., 40S, small ribosomal subunits; 60S, large ribosomal subunits; and 2 × 80S, low molecular weight polysomes consisting of two ribosomes; h denotes human and y signifies yeast ribosomal subunits/polysomes. To the left of each electrophoretogram are denoted the positions of rRNAs in corresponding gels (note, the 5S rRNA label denotes the collective positions of 5.8S rRNA, 5S rRNA and tRNAs in gels).