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. 2020 Feb 13;21(4):1254. doi: 10.3390/ijms21041254

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Typical distribution and abundance of ribosome- and polysome- ribonucleoprotein complexes in polysome profiles as determined by qRT-PCR analysis. Data are derived from four biological replicates of: (a) HEK-293 cell line (note, the same samples were analysed in Figure 2c); (b) 200 mouse NEBD-stage oocytes used for RNA-seq; (c) 200 zygotes and (d) 200 two-cell embryos. 18S and 28S contents in each fraction are displayed as percentages of the respective fraction compared to the whole polysome profile; error bars indicate ±SD of biological replicates. Note, qRT-PCR-based visualization of individual replicate/source polysome profiles are included in Figure S4.