Figure 3.

Preparation of Grb7 constructs. (A) The domain boundaries of Grb7 full-length, Grb7-RA-PH and Grb7-SH2 used for the preparation of GST-tagged proteins are shown. (B) SDS-PAGE analysis of the purified GST Grb7 constructs used for SPR experiments. (C) Schematic depicting the SPR experimental design. The GST Grb7 construct is captured on the surface of the biosensor chips via anti GST antibody. CaM is flowed across the surface of the chip under controlled buffer conditions, enabling interactions to be detected via SPR.