Figure 5.

Functional characteristics of endothelial mitochondria isolated from control and ATOR-exposed cells. Maximal (phosphorylating or uncoupled) respiration with various respiratory substrates (A). Cytochrome c oxidase (COX, CIV) activity (B). Other functional parameters during succinate, malate, and a mixture of malate and succinate oxidation under nonphosphorylating conditions (in the absence of ADP) and under phosphorylating conditions (in the presence of ADP) (C–E). H₂O₂ production with various substrates (C). Percentage relative to the control mitochondria oxidizing succinate alone (plus rotenone; 100%). mQ10 reduction level (mQH₂/Qtot) (D). Mitochondrial membrane potential (mΔΨ) (E). Mean ± SD; n = 4-8; p < 0.05 (*), p < 0.01 (**), comparison vs. control values.