Figure 3.

Loss of antiaggregation effect due to DNAJB6 mutations. Various DNAJB6b constructs were tested in a filter trap assay for their ability to prevent the aggregation of polyQ huntingtin. Similarly to the previously tested p.F89I and p.F93L, both p.P96R and p.F100I mutations showed impaired antiaggregation activity. Wild-type DNAJB6a (a wt) and DNAJB6b (b wt) serve as negative and positive controls, respectively. The graph shows mean ± S.D. of eight to nine replicate transfections from three separate experiments. Asterisks indicate statistically significant differences to b wt according to the Mann–Whitney U test (** p < 0.01, *** p < 0.001).