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. 2020 Feb 19;21(4):1390. doi: 10.3390/ijms21041390

Figure 4.

Figure 4

Effects of chilling stress (18 °C and 12 °C) on activities of enzymes involved in chlorophyll biosynthesis. Glutamate-1-semialdehyde transaminase (GSA-AT, A), ALA dehydratase (ALAD, B), Mg-chelatase (C), protochlorophyllide oxidoreductase (POR, D) activities of control (28 °C) and chill-stressed (18 °C and 12 °C) rice seedlings after 0 h, 0.5 h, 12 h, and 48 h greening. Six day old etiolated seedlings were treated with 18 °C or 12 °C chilling stress. Seedlings were harvested at 0 h, 0.5 h, 12 h, and 48 h of greening and their activities of enzymes involved in chlorophyll biosynthesis were measured. The activities of enzymes at 28 °C after 48 h of light exposure were defined as 100%. Values are means ± SD from three independent biological replicates. Different letters indicate significant differences according to Duncan’s multiple range tests at p < 0.05. Each data point is the average of three replicates. The error bars represent SD.