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. 2020 Feb 19;21(4):1390. doi: 10.3390/ijms21041390

Figure 9.

Figure 9

Effects of chill-stressed (18 °C and 12 °C) on H2O2 (A,C), O2.− (B,D), MDA content (E), and EL (F) of rice seedlings. Histochemical detection (A,B), content of H2O2 (C), O2.− (D), MDA (E), and EL (F) of control (28 °C) and chill-stressed (18 °C and 12 °C) rice seedlings after 0 h, 0.5 h, 12 h, and 48 h greening. Six day old etiolated seedlings were treated with 18 °C or 12 °C chilling stress. Seedlings were harvested at 0 h, 0.5 h, 12 h, and 48 h of greening and their ROS levels were measured. Values are means ± SD from three independent biological replicates. Different letters indicate significant differences according to Duncan’s multiple range tests (p < 0.05).