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. 2020 Feb 22;21(4):1486. doi: 10.3390/ijms21041486

Figure 4.

Figure 4

GAL3 deletion does not modulate chondrocyte anabolism. (A) Immunohistochemistry of expression of type II collagen and aggrecan in knee cartilage of WT and Gal3−/− cartilages after MNX (n = 5 of WT and Gal3−/− mice). Scale bars: 100 µm (B) qRT-PCR analysis of Coll II, Acan and Sox9 mRNA expression in cultured chondrocytes from articular cartilages of Gal3−/− and WT newborn mice with and without IL-1β stimulation (10 ng/mL) (201-LB/CF, R&D Systems, Lille, France) (n = 5 independent experiments). (C) qRT-PCR analysis of Lgals1, Lgals2, Lgals4, Lgals7, Lgals8, Lgals9 and Lgals12 expression in cultured chondrocytes from articular cartilage of Gal3−/− newborn mice (n = 5 independent experiments). Two-tailed Mann–Whitney U test between WT and Gal3−/−.