Figure 2.

Replicative spread of GS4-GFP in glioma cells in culture and in intracerebral gliomas in vivo. (A) GS4-GFP-transduced RG-2 cells (5%) and uninfected RG-2 cells (95%) were mixed and seeded onto culture plates. At various time points after cell mixing, the cell populations were analyzed for GFP expression. X-axis: days after cell mixture. Y-axis: % of cells expressing GFP. (B) GS4-GFP (2 × 10⁴ TU) was injected into a pre-established intracerebral RG-2 tumor model in Fischer 344 rats. The spread of GS4-GFP in tumors was examined by quantification of GFP expression at 13 (n = 6) and 19 days (n = 3) after viral vector inoculation.