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Effect of basolateral side addition of both TNFα and IFNγ (10 ng/mL) for 72 h on A) Papp of apically-added FITC-Dextran 4kDa (FD4) across HIO-derived epithelium obtained over 120 min. B) MTS viability indicated no cytotoxic effect from exposure to TNFα and IFNγ. Two-way ANOVA with Bonferroni’s multiple comparisons; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with respective control. Each value represents the mean ± SEM, n = 3, with 3 independent replicates.
