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. 2020 Feb 21;21(4):1490. doi: 10.3390/ijms21041490

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Axitinib-dependent ATM phosphorylation in HUVECs was not altered by GBM cells coculture. pATM immunostaining was performed on HUVECs cocultured with U87 (a,b, left panel) or A172 (a,b, right panel) GBM cells. CTR: sham-treated HUVECs; AXI: Axitinib-treated HUVECs; TW: sham-treated HUVECs cocultured in transwell with GBM cells for 48 h; TW AXI: HUVECs cocultured in transwell with GBM cells and treated with Axitinib. Immunofluorescence was performed at the end of the 1h Axitinib pulse. Mean values and standard deviation were generated from at least three biological replicates. Magnification: 20×; scale bar 50 µm.