Table 2.
Target | Primer | Sequence (5′-3′) | Product Size | Accession Number |
---|---|---|---|---|
Ligands | ||||
cGHRH | Fwd | TAC CTG AGT GGG AGC TGA TC | 704 | >NM_001040464.1 |
Rev | CAT CAG TCT CCA GCT GGT CA | |||
cTRH | Fwd | ATT AAA CAT GCC TCT GCC ACA | 600 | >XM_025154454.1 |
Rev | AAA CAA TTA CTT TCT CAT TCC TCT G | |||
cGhrelin | Fwd | CATACAGCAACAAAAGGATAC | 272 | >NM_001001131.1 |
Rev | TGTGGTTGTCCTTCAGCT | |||
cSST | Fwd | CACCTGTCCTCCCCATCCAC | 232 | >NM_205336.1 |
Rev | CGGAGTGCATGTCACGCAAG | |||
cGH | Fwd | CGCACCTATATTCCGGAGGAC | 128 | >NM_204359.2 |
Rev | GGCAGCTCCATGTCTGACT | |||
cBAFF | Fwd | GATCTCAGCTTGGTGACATTAT | 140 | NM_204327.2 |
Rev | TTAGCTCTTCTTCGTGGTATTG | |||
Receptors | ||||
cGHRH-R | Fwd | GCTGGTCAGAGCCATTCCCTT | 177 | >NM_001037834.2 |
Rev | AGCGTAGCCTCCTGAATGCCA | |||
cTRH-R | Fwd | ATGCCCTATCGAACACTGGT | 177 | >NM_204930.1 |
Rev | ATGGCAGTTGCAGAGTTTCCT | |||
cGHS-R | Fwd | TGGCCTTCTCCGACCTGCT | 180 | >NM_204394.1 |
Rev | TGGCGACGTACCGCTCCAC | |||
cSSTR-1 | Fwd | CCGTGGCTAAGATGGTCAACCT | 189 | >NM_001113167.1 |
Rev | GCAGCAAGAAGCCCATCAGGA | |||
cSSTR-2 | Fwd | GGCACCGGTATGTAGGGAGTC | 191 | >XM_015279869.2 |
Rev | ATGCGTGCTGCCACATGGG | |||
cSSTR-3 | Fwd | GGCACCGGTATGTAGGGAGTC | 172 | >XM_015286025.2 |
Rev | ATGCGTGCTGCCACATGGG | |||
cSSTR-4 | Fwd | GGCCATGTTCGTTGTCTGCT | 181 | >XM_015283378.2 |
Rev | GCCGTGGAAAGAGTGCCGGA | |||
cSSTR-5 | Fwd | CCGCTACCTGGCAGTAGTTCA | 156 | >XM_015294246.2 |
Rev | TTGCAGGTGTGAAAGTCCTCC | |||
cGH-R | Fwd | ACTTCACCATGGACAATGCCTA | 181 | >NM_001001293.1 |
Rev | GGGGTTTCTGCCATTGAAGCTC | |||
Reference genes | ||||
cGAPDH | Fwd | TGTGGAGAGATGGCAGAG | 154 | >NM_204305.1 |
Rev | GTCAGGTCAACAACAGAGAC | |||
c18S | Fwd | CTCTTTCTCGATTCCGTGGGT | 100 | >XR_003078044.1 |
Rev | TTAGCATGCCAGAGTCTCGT |