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. 2020 Feb 21;21(4):1492. doi: 10.3390/ijms21041492

Figure 3.

Figure 3

Over-expression of miR-378 increases the proportion of ASC that express FABP4 in an adipogenic assay. Late passage ASC were transfected with a miR-378 mimic and subjected to an in vitro adipogenic differentiation assay. Panel (A) shows a representative image of ASC subjected to the adipogenic differentiation assay taken at 20 X magnification. A single lipid-droplet containing cell staining positive for FABP4 expression is shown below in a cropped and enlarged image (labelled ‘zoom’). FABP4 positive cells are stained green and nuclei are stain blue. Panel (B) shows representative images from the adipogenic differentiation assay taken at day seven for quantification purposes for three donors (D1–D3) transfected with the miR-378 mimic or a scrambled control. The graph in (C) represents the relative expression of miR-378 in cells one week post transfection. Panel (D) shows the percentage of cells that stained positive for FABP4 expression after quantification at day 7 and 14 of the adipogeneic assay, n = 6. * denotes p value < 0.05.