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. 2020 Feb 19;21(4):1414. doi: 10.3390/ijms21041414

Figure 1.

Figure 1

Prediction and confirmation of signal peptides of CLIBASIA_03915 and CLIBASIA_04250. (A) The signal peptides of CLIBASIA_03915 and CLIBASIA_04250 were predicted by SingalP 3.0 and SingalP 5.0. The cleavage site of CLIBASIA_03915 was between 20–21 aa (SignalP 5.0) or 24-25 aa (SignalP 3.0). SignalP 3.0 and SignalP 5.0 predicted the same cleavage site of CLIBASIA_04250 (20-21 aa). The arrows indicated the cleavage sites. (B) The signal peptides of CLIBASIA_03915 and CLIBASIA_04250 were confirmed by phoA assay. The E. coli cells harboring the recombinant plasmids along with the negative control pJDT1 were streaked on Luria–Bertani (LB) plates amended with 5-bromo-4-chloro-3-indolylphosphate (BCIP) and sodium phosphate. The negative control pJDT1 did not cause the color change. The E. coli cells expressing the recombinant plasmids turned to blue.