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. 2020 Feb 22;21(4):1495. doi: 10.3390/ijms21041495

Figure 2.

Figure 2

NTA size distribution profiles of EVs released from LN18 and LN229 cells following PAD isozyme-specific inhibitor treatment for 1 h and EV characterisation. Representative NTA profiles of LN18 cells following 1 h PAD inhibitor treatment (A–D): (A) Control DMSO treated cells; (B) PAD2 inhibitor treated cells; (C) PAD3 inhibitor treated cells; (D) PAD4 inhibitor treated cells. Representative NTA profiles of LN229 cells following 1 h PAD inhibitor treatment (E–H): (E) control DMSO treated cells; (F) PAD2 inhibitor treated cells; (G) PAD3 inhibitor treated cells; (H) PAD4 inhibitor treated cells. (I) Western blotting analysis (WB) showing that EVs isolated from LN18 and LN229 cells are positive for the EV specific markers CD63 and Flot-1; β-actin is absent from the EVs but present in the cells. (J) Transmission electron microscopy (TEM) images showing characteristic EV morphology for EVs isolated from both cell lines; the scale bar indicates 50 µm. In the NTA curves the black line represents the mean of the 5 repetitive readings per individual sample and the red line represents standard error (+/−) between those same 5 readings per sample. Each treatment group was measured in 3 biological replicates.