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. 2020 Feb 18;21(4):1379. doi: 10.3390/ijms21041379

Figure 4.

Figure 4

Secretory profile of B16F10 melanoma cells and immunological consequences in dendritic cells. (A–C) Multiplex cytokine analysis (A) and HSP70 ELISA of supernatants of murine B16F10 cells receiving plasma (10 s at 0 h), fractionated radiotherapy (at 0, 24, and 48 h), or both, collected at 24 h after the last radiation dose (B), or mono or combination single radiotherapy with plasma (C); (D–J) JAWS murine dendritic cells cultured for 96 h with supernatants of B16F10 cells, and multicolor flow cytometry mean fluorescent intensity (MFI) analysis of activation markers CD11b (D), CD40 (E), CD80 (F), CD83 (G), CD86 (H), and MHC class II on viable cells (I) as well as percentage of MHC class II and CD83 double positive cells (J). Data are representative and mean + SE of three experiments. Statistical analysis was performed by one-way ANOVA. Gy = gray, Utr = untreated, PL = plasma, Rad = radiotherapy; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.