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. 2020 Feb 24;21(4):1548. doi: 10.3390/ijms21041548

Figure 2.

Figure 2

MPNST cell viability after combinatory treatment with MK-2206 and AZD8055 in vitro. MPNST cells were seeded in a 96-well-plate at a density of 5000 cells per well. Relative cell viability (normalised to control) of S462sp (A) and S1507.2 (B) cells under varying concentrations of combined MK-2206 and AZD8055 treatment was measured in an XTT assay after 72 h. The mean values with standard deviation are shown. Statistical significance was analysed with a one-way ANOVA with Dunnett’s multiple comparisons test and compared to the vehicle substance dimethyl sulfoxide (DMSO) (* p ≤ 0.05; *** p ≤ 0.001; **** p ≤ 0.0001). Combination indices (CI) were calculated based on the XTT assay (Figure 1 and Figure 2) according to the Chou and Talalay method and plotted against their respective fractional effect (Fa) on cell viability (very strong synergism CI < 0.1; strong synergism CI 0.1–0.3; synergism CI 0.3–0.7; moderate synergism CI 0.7–0.85; slight synergism CI 0.85–0.90; nearly additive CI 0.9–1.10; antagonistic CI > 1.1). The data for dual targeting with MK-2206 and AZD8055 on S462sp (C) and S1507.2 (D), compared to single treatment, are shown.