Figure 2.

TCEE induces endothelial NO synthase (eNOS) activation in ECs. ECs were treated with TCEE (2 μg/mL) for the indicated times. (A–D) Cells were lysed and subjected to Western blot analysis for quantifying the levels of phosphorylated eNOS (at Thr495, Ser617, Ser635, and Ser1177) or total eNOS protein. Data are mean ± SEM from 5 independent experiments. * p < 0.05 vs. vehicle-treated group.