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. 2020 Feb 24;21(4):1532. doi: 10.3390/ijms21041532

Figure 3.

Figure 3

Akt-CaMKII-AMPK signaling is involved in the TCEE-mediated enhancement of NO bioavailability in ECs. (A–C) ECs were treated with TCEE (2 μg/mL) for the indicated times. Western blot analysis of phosphorylated or total of Akt, CaMKII, and AMPK and α-tubulin. (D) ECs were pretreated with LY294002 (10 μM), compound C (C.C., 10 μM), or KN62 (10 μM) for 2 h, and then with TCEE (2 μg/mL) for 24 h. Griess assay of the nitrite level in the culture medium. Data are mean ± SEM from 5 independent experiments. * p < 0.05 vs. vehicle-treated group; # p < 0.05 vs. TCEE-treated group.