Figure 5.

The signaling cascade involved in the TCEE-induced promotion of EC proliferation and migration. ECs were pretreated with LY294002 (10 μM), compound C (C.C., 10 μM), or KN62 (10 μM) for 2 h, then with TCEE (2 μg/mL) for 8 h. (A) MTT assay of EC proliferation. (B,C) EC migration was evaluated by the trans-well assay after TCEE treatment. The number of migrating cells was counted compared to the case for the vehicle-treated cells, and the images were generated by microscopy. Data are mean ± SEM from 5 independent experiments. * p < 0.05 vs. vehicle-treated group; ^# p < 0.05 vs. TCEE-treated group.