Figure 7.

TCEE inhibits the TNFα-induced adhesion of monocytes onto ECs and the protein expression of adhesion molecules in ECs. ECs were pretreated with TNFα (10 ng/mL) or the vehicle for 12 h, followed by treatment with L-NAME (400 μM) for 1 h and/or TCEE (2 μg/mL) for another 18 h. (A–C) Cellular lysates were subjected to Western blot analysis for quantifying the protein expression of ICAM-1, VCAM-1, and α-tubulin. (D,E) BCECF-AM-labeled human monocyte THP-1 cells (1 × 10⁵) were added and incubation with ECs for 1 h. The cellular lysates were subjected to fluorometry and photo-micrographed. Data are mean ± SEM from 5 independent experiments. * p < 0.05 vs. vehicle group; ^# p < 0.05 vs. TCEE-treated group; ^$ p < 0.05 vs. TNF-α +TCEE-treated group.