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. 2020 Feb 23;21(4):1524. doi: 10.3390/ijms21041524

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Experimental design of two combined online and offline 3D-LC platforms for proteomic applications. (A) Large-scale proteome quantification of human hepatocellular carcinoma and normal liver tissues using high-pH RPLC pre-fractionation and online SCX−RP 2D nanoflow LC−MS/MS analysis. Reprinted with permission from [79], Copyright (2014) American Chemical Society. (B) Mouse brain proteome investigation using online SCX-RPLC fractionation and capillary zone electrophoresis (CZE)-MS/MS analysis. Reprinted and modified from [51], Copyright (2018) with permission from Elsevier.