Table 2.
Performance of some online 3D-LC systems.
| 3D-LC Setup | Sample (Amount) | MS | Identified Proteins * | Identified Unique Peptides | Fraction Number | MS Time (h) | Year, Reference |
|---|---|---|---|---|---|---|---|
| RP-SCX-RP | Saccharomyces cerevisiae (200 µg) | LCQ Deca XP | ~1900 | - | 60 | 140 | 2005, [76] |
| RP-SAX-RP | Saccharomyces cerevisiae (5 µg) | Orbitrap XL | 4004 | 26,468 | 101 | 202 | 2011, [80] |
| 3821 | 25,091 | 51 | 102 | 2011, [80] | |||
| RP-SAX-RP | Murine embryonic stem cells (25 µg) | Orbitrap XL | 11,352 | 211,535 | 20 | 580 | 2013, [82] |
| RP-SCX-RP | PC12 cell (100 µg) | AB Sciex QSTAR XL QTOF | 6345 | 97,309 | 12 | 24 | 2015, [83] |
* Proteins identified with at least two peptides.